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PVDF Western Blotting Membrane

Immunoblotting test is also known as western blot, which is a hybrid technology combining high-resolution gel electrophoresis and immunochemical analysis.

Western blot has the advantages of large analytical capacity, high sensitivity and strong specificity. It is the most common method to detect the characteristics, expression and distribution of proteins, and is widely applicated in:


Study on Gene Expression at Protein Level
Antibody Activity Test
Early Diagnosis of Disease



Advantages

Smooth and flat surface, not easy to curl
High mechanical strength, easy to be stripped and reprobed
Evenly distributed pore size, high protein mobility, clear results
High sensitivity to ensure successful detection of low abundance protein



Application

Western Blot

Adsorption Analysis

Amino Acid Analysis

N-terminal Protein Sequencing

Spot and Slit Printing Detection

Glycoprotein Coloration

Lipopolysaccharide Analysis



   

Performance Comparison of Immunoblotting Membrane

Membrane MaterialNCPVDF
Protein binding capacity μg/cm2LowHigh
Mechanical strengthFragileDurable
Chemical compatibilityLimited

Compatible with most organic solvents,
acids and weak bases

Whether activation is requiredNoMethanol activation required
Detection

Chemiluminescenc
Fluorescence detection
Conventional dyeing

Chemiluminescence
Fluorescence detection
Conventional dyeing
Coomassie blue staining

ReprocessingUnsuitableSuitable
Heat resistancePoorGood



  • Specification
  • Performance
  • Ordering Information

Comparison of PVDF Transfer Membrane


Company / ModelM CompanyT Company

Cobetter
TS2590L

Cobetter
TS2590H

Application

Common
immunoblotting 

Common
immunoblotting 

Especially suitable for low
abundance protein detection

Common
immunoblotting 

MaterialPVDFPVDFPVDFPVDF
Pore Size / μm0.450.450.450.45
Thickness / μm12712791115
Test Method

Chemiluminescence
 Substrate Coloration

Results of 45kDa
Proteins

PVDF western cbt 00 (1).pngPVDF western cbt 00 (12).pngPVDF western cbt 00 (11).pngPVDF western cbt 00 (10).png

Results of 35kDa
Proteins

PVDF western cbt 00 (9).pngPVDF western cbt 00 (8).pngPVDF western cbt 00 (7).pngPVDF western cbt 00 (6).png

Results of Proteins
less than 30kDa

PVDF western cbt 00 (5).pngPVDF western cbt 00 (4).pngPVDF western cbt 00 (3).pngPVDF western cbt 00 (2).png




According to the chemiluminescence test results, the 0.45μm hydrophobic PVDF transfer membrane developed by Cobetter can achieve similar protein transfer effect as competitor’s products.

The 0.2 micron model is under development.


Ordering Information

ModelPore SizeLength (mm)Width (mm)
TS2590L0.45 μm2603300

High sensitivity model, suitable for detection of low abundance protein..

TS2590L297210
TS2590L210148
TS2590L200200
TS2590L150150
TS2590L8470
TS2590H0.45 μm2603300General model, relatively high sensitivity, suitable for detection of high abundance protein
TS2590H297310
TS2590H210148
TS2590H200200
TS2590H150150
TS2590H8470


* More sizes can be customized.

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