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Home Applications Biopharmaceutical Manufacturing Monoclonal Antibody Ultrafiltration & Concentration

Ultrafiltration is an important step to separate, purify and concentrate protein solutions in biopharmaceutical manufacturing, improving production capacity and recovery rate. The retention capacity of ultrafiltration membranes are characterized by the molecular weight cut-off (MWCO) of the target protein, typically ranging from 1kDa to 1000kDa.



Determine the MWCO of Ultrafiltration Membrane

  • To have the target macromolecule permeate the membrane, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times bigger than the MW of target macromolecule.
  • To have the target macromolecule to be retained, generally choose an ultrafiltration membrane with a MWCO that is 3-5 times smaller than the MW of target macromolecule.


Choose the Right TFF Cassettes


Polyethersulfone(PES) Regenerated Cellulose(RC)
Features pH range: 1-14
High flux, very low protein binding
Natural Hydrophilic
Resistant to organic solvents
Suitable for high concentration formulation
MWCO 1, 3, 5, 8, 10, 30, 50, 100, 300, 500, 1000 kDa 1, 2, 3, 5, 8, 10, 30, 50, 100, 300 kDa
Typical Applications Oligonucleotide, Peptide, Proteins, Growth factor, FVIII,Recombinant proteins,mAb, Vaccines, Viral vectors Peptide, Insulin, Growth factor, Recombinant proteins,mAb, Collagen, LNP, Exosome, Viral vectors
Filtration Area 100cm2, 200 cm2, 0.11 m2, 0.46m2, 0.5 m2,  2.33 m2,  2.5 m2


Choose the Right Hollow Fiber TFF


Fiber ID Effective Length Batch Volumes Feed/Retentate Connectors Permeate Connectors MWCO
Mini 0.5mm
1.0mm
30cm
60cm
<600mL Female Luer Female Luer 3 kDa
5 kDa
10 kDa
30 kDa
50 kDa
100 kDa
300 kDa
500 kDa
750 kDa
Minilab 0.5mm
1.0mm
30cm
60cm
<2L Female Luer Female Luer
Lab 0.5mm
1.0mm
30cm
60cm
2L-16L 0.5'' TC 3/16'' HB
Pilot 0.5mm
1.0mm
30cm
60cm
110cm
15L-80L 1.5'' TC 0.5'' TC
Process 0.5mm
1.0mm
30cm
60cm
110cm
75L-800L 1.5'' TC 1.0'' TC
Maxi 0.5mm
1.0mm
30cm
60cm
110cm
800L-1500L 2.0'' TC 1.5'' TC

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