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Nylon Membrane

Cobetter Nylon membrane, with its natural hydrophilicity, can be easily wetted by water. There is a PET support layer in the middle of the membrane, which gives the Nylon membrane high mechanical strength. The symmetric nylon layer produced by non-solvent induced phase separation exhibits high porosity and large surface area, which can provide abundant reaction sites for diagnostic reagent reactions. The flat and smooth surface of both sides, as well as the uniform pore size makes the nylon membrane a printing paper-like texture. Nylon membrane is resistant to most common solvents such as ethanol, acetone, formaldehyde, etc, except for concentrated acid. In addition to neutral membranes without chemical charges, the surface of Nylon membrane can covalently bind different charges through coating processes, such as negative membranes with carboxyl groups and positive membranes with quaternary ammonium groups. Different types of electrical properties adsorb or repule different detection substances, improving the resolution of the test.
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Description

Type

Neutral Nylon Membrane

No additional charge is added and the membrane is basically electrical neutral. Its net charge can transform in different pH environments, resulting in different electrostatic adsorption. By regulating the pH of the reaction environment the membrane would show good specific adsorption and test resolution can be improved. UV crosslinking or baking can also be used to coat the film with additional reagents, such as positive charge modification and negative charge modification.

Positively Charged Nylon Membrane

The membrane contains a large number of quaternary ammonium groups. Through electrostatic interactions, positively charged nylon membranes can be used to adsorb, filter or purify negatively charged substances such as nucleic acids, proteins, endotoxins, etc.

Negatively charged nylon film

The negatively charged membrane carrying carboxyl groups provides electrostatic adsorption for positively charged substances. Besides the carboxyl groups can be used to covalently graft special reagents. For example with the aid of EDC and other diimide reagents, covalently bind the amino groups of pre designed gene probes to the carboxyl groups on the membrane. The binding sites of the probes can specifically bind the labeled PCR products of the target genes, and generate specific patterns in the subsequent coloration step for diagnosis results.

Features

  • Natural Hydrophilic
  • Symmetric structure
  • Smooth surface
  • High mechanical strength
  • Good chemical stability
  • Available in positive, negative or neutral charged

Applications

  • Dry chemistry testing
  • Southern blotting
  • Reverse dot blot
  • ELISA
  • oryzaeDNA 
  • Affinity purification
  • Cloning/phage selection
  • Solvent filtration

Specifications

Type Pore Size
[μm]
Bubble Point
[kPa]
(water 20°C)
Thickness
[μm]
Water Flow Rate
[s/50ml *12.68 cm2, 20°C water]
Charge Charge Density
[mol/cm2]
N66X-YFZCXSYH 0.45 μm ≥170kPa 135±35 ≤45s @-30kPa Neutral /
N66X-YFPCZC0.45Y 0.45μm ≥170kPa 160±35 ≤45s @-30kPa Positive >300
N66X-YF3FDZC0.45Y 0.45μm ≥170kPa 150±35 ≤55s @-30kPa Negative 250-300

Ordering Information

Sheets: sizes can be customized. (The width is less than 265mm)
Rolls: maximum width is 265mm and maximum length is 300m. 

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